Se diseñaron los biorreactores, utilizando principios de similitud; para la producción del alcohol etílico, se diseñó un biorreactor del tipo Lecho Empacado . Inmovilización de levaduras en residuos lignocelulósicos para la producción de etanol en biorreactor de lecho empacado. En las fermentaciones realizadas en los biorreactores de lecho empacado con el biocatalizador (soporte + levaduras), se logró obtener un aumento en la.

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We used glucose as a carbon source to establish the reference conditions and we made the fermentations with commercial sucrose. The productivity reached was In the last two decades there has been an increase of research focused on developing new technologies for ethanol production from renewable sources, and the efforts have focused towards the development of much more efficient production processes [1- 3].

Results and discussion Due to heterogeneity in nature and origin of agro-industrial waste used in the experiment, and to avoid interference in the subsequent process of cell immobilization, it was necessary to establish a cleaning treatment and adapting materials. These materials empscado subject to a conditioning process that involved the implementation of a protocol for cleaning, drying and size reduction.

Releases to the environment in Colombia. As a carbon source, we evaluated sucrose and glucose syrup obtained from cassava flour.

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The choice of the carrier sizes and flow rates for immobilization in fermentation conditions within the studied ranges can be made by considering the best operating conditions. In this work, we evaluated the continuous production of ethanol in packed-bed reactors with yeast cells immobilized on wood shaving, cane bagasse, corn leave, and corn cob lignocellulosic waste.

The result of the study showed that the material in which the biggest amount of cells was immobilized was the sugar cane bagasse, and we obtained a value of 0. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.

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Two different types of bioreactors were designed, using principles of similarity.

Revista Facultad de Ingeniería

The aim of this study is to establish a process of cell immobilization on lignocellulosic waste and their potential use in the production of ethanol. Medir la masa combinada del filtro y pesar barco. An experimental design was conducted to determine the effects of the size of lignocellulosic materials on cell immobilization and to establish the effects of the flow rate in emoacado immobilized cells when the fermentation is performed in packed bed bioreactors.

System Setup and Basic Operation …. However, to achieve better results and be able to implement this system at industrial level, it is necessary wmpacado optimize the process to establish the best concentrations of sugars, inoculum size and biocatalyst quantity appropriate for the bioreactor, improving the yields and making the process competitive with the actual existent ethanol production technology. However, an industrial implementation of this process requires further optimizations that allow for one to increase the amount of cells immobilized on the carriers, as well as improvements in the operational conditions of the process.

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Initially, the cells have more affinity for folds and rough surfaces. Figures 4 and 5 show the results obtained for the continuous fermentation carried out with sucrose. The reduced sugars in the culture medium were determined using the technique of DNS [20]. As observed in Fig.

Modelado de reactores de lecho empacado en COMSOL®

Show full item record. The proposed hypothesis is that these materials are appropriate matrices for the immobilization of yeast cells, which allow high cellular activity, lasting stability and they enable continuous operation for the production of ethanol.

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If that doesn’t help, please let us know. There are also demanding requirements for materials used as carriers, since chemical, physical and biological stability has to be ensured, and the carriers and biorrewctor technique must be low cost and easy to implement on an industrial scale. In the experimental design, 15 treatments were evaluated by combining three sizes 3. A Cole Parmer peristaltic pump model was used, operating at a flow of 0. Although in this process high temperatures are applied to reduce significantly the initial microbial load of materials, we found the need of sterilization before they were used in the immobilization.

The concentration of alcohol was determined by HPLC.

Prior to the start of the continuous process, we performed a batch stage of 12 h. The protocol developed was described in the methodology biomass quantification protocol. Journal Institute of Brewingpp. The characterization made it possible to establish the microscopic structural differences between the four materials, as well as the differences in composition of lignin, cellulose, hemicelluloses and ash.

Esquema de un biorreactor de alta densidad HDBR no t escala o. At the output of the reactor we obtained concentrations from For other languages click here. The established time of residence t was 3 h, and the reactor was operated this way for 6 times of residence 18 h. JavaScript is disabled for your browser.

De-inmobilization was performed by mechanical agitation at rpm for 24 hours at room temperature.